The myo-Inositol Assay Kit is a reliable and accurate enzymatic UV-method for the specific measurement and analysis of myo-inositol in foods, dairy products and various other materials.
Phytic acid content of samples with very low levels of free myo-inositol can also be determined using K-INOSL. This can be achieved by measuring the amount of myo-inositol released after de-phosphorylation of phytic acid with the enzymes phytase and alkaline phosphatase, as used with the Megazyme Phytic Acid/Total Phosphorus Assay Kit (K-PHYT).
Not suitable for the determination of myo-inositol in baby formula.

Colourimetric method for the determination of myo-Inositol
in various sample matrices

Principle:
(myo-inositol dehydrogenase)
(1) myo-Inositol + NAD+ →
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+

(diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan

	Kit size:  50 assays Method:  Spectrophotometric at 492 nm Reaction time:  ~ 10 min Detection limit:  0.8 mg/L Application examples: Animal feeds, food, baby milk formulation and other materials Method recognition:  Novel method

Advantages

• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Only enzymatic kit available
  
• Rapid reaction
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included