The D-Isocitric Acid test kit is for the specific and rapid measurement and analysis of D-isocitric acid in fruit juices.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of D-Isocitric Acid in foodstuffs

Principle:
(isocitrate dehydrogenase)
(1) D-Isocitric acid + NADP+ → 2-oxoglutarate + CO2 + NADPH + H+

(pH 9-10)
(2) D-Isocitric acid ester + H2O → D-isocitric acid + alcohol

(pH 9-10)
(3) D-Isocitric acid lactone + H2O → D-isocitric acid

	Kit size:  100 assays (manual) / 1000 (microplate) 
                                         / 1000 (auto-analyser) Method:  Spectrophotometric at 340 nm Reaction time:  ~ 3 min Detection limit:  0.35 mg/L Application examples: Fruit juices, fruit products, soft drinks and other materials (e.g. biological
cultures, samples, etc.) Method recognition: Methods based on this principle have been accepted by EN, NEN, NF,
DIN, GOST, IFU and AIJN

Advantages

• Very competitive price (cost per test)
  
• All reagents stable for > 2 years after preparation
  
• No wasted isocitrate dehydrogenase solution (stable suspension supplied)
  
• Very rapid reaction
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats