The Glucomannan test kit is suitable for the measurement and analysis of Glucomannan in plant products and food.

UV-method for the determination of Glucomannan in plant
products, foodstuffs and other materials

Principle:
(β-mannanase)
(1) Ac-Glucomannan + H2O → Ac-glucomanno-oligomers

(pH 12.5)
(2) Ac-Glucomanno-oligomers + H2O → glucomanno-oligomers
+ acetate

(β-glucosidase + β-mannosidase)
(3) Glucomanno-oligomers + H2O → D-glucose + D-mannose

(hexokinase)
(4) D-glucose + D-mannose + ATP → G-6-P + M-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphomannose isomerase) (phosphoglucose isomerase)
(6) M-6-P → F-6-P → G-6-P

	Kit size:  50 assays Method:  Spectrophotometric at 340 nm Total assay time: 120 min Detection limit:  1-100% of sample weight Application examples: Jelly sweets, cosmetics, food gums and other materials Method recognition:  Novel method 

Advantages

• Very cost effective
  
• Only enzymatic kit available
  
• Simple format
  
• All reagents stable for > 2 years after preparation
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included